Storage: Store at 2–8°C. DO NOT FREEZE.
NexaFect™ DNA Transfection Reagent is a synthetic, non-liposomal reagent engineered for high-efficiency plasmid DNA (pDNA) delivery, high cell viability, and robust gene expression. Optimized for both adherent and suspension cells, it provides reliable and reproducible performance across a broad range of cell lines.
Designed for versatility, NexaFect™ DNA supports transient transfection, stable cell line generation, recombinant protein expression, high-throughput screening, and plasmid transfection for viral vector production, including lentiviral and adenoviral vectors. Its excellent serum compatibility enables direct transfection in complete growth medium containing serum, eliminating unnecessary medium changes and streamlining the transfection workflow.
Key Advantages
1. High Efficiency & Excellent Cell Viability
Delivers high transfection efficiency while maintaining excellent cell viability. Compared with conventional PEI-based reagents, NexaFect™ DNA offers lower cytotoxicity and reduced apoptosis, helping preserve cell health following transfection.
2. Serum-Compatible, No-Medium-Change Workflow
Transfect directly in complete, serum-containing medium without switching to serum-free formulations or changing the medium after transfection. This streamlined workflow reduces hands-on time, minimizes cell stress, and improves experimental reproducibility.
3. Broad Cell Line Compatibility
Delivers reliable and reproducible performance across a broad range of adherent cell lines, including HeLa, 293T, CHO, Vero E6, LX-2, and MDA-MB-231 cells.
4. Versatile Applications
From routine transient transfection and high-throughput screening to large plasmid delivery, stable cell line generation, recombinant protein expression, and lentiviral and adenoviral vector production, NexaFect™ DNA provides a versatile solution for diverse research workflows.
5. Excellent Stability & Lot-to-Lot Consistency
A stable formulation supports ambient-temperature shipping and convenient storage at 2–8°C, with minimal tendency to aggregate. Consistent lot-to-lot performance helps ensure reliable and reproducible experimental results.
Transfection Efficiency of NexaFect™ DNA vs. Leading Transfection Reagents
Transfection Performance of NexaFect™ DNA vs. Leading Transfection Reagents
| Transfection Reagent | CHO | HeLa | MuM-2B | LX-2 |
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| NexaFect™ DNA |  |
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| Competitor |  |
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Optimization of the NexaFect™ DNA Reagent-to-pDNA Ratio
