Resins for Solid Phase Peptide Synthesis: Application Guide
Resins for Fmoc SPPS
|
Products |
Resins |
Attachment |
Cleavage |
|
|
Peptide Acid |
Wang Resin |
DIC/HOBt/DMAP |
95%TFA/Scavenger |
|
|
4-Benzyloxybenzyl bromide Resin |
Cesium salt or KF |
95% TFA/Scavenger |
||
|
Protected Peptide Acid |
2-Chlorotrityl Chloridde Resin |
DIPEA |
EtOH/TFE/DCM(1:2:7) 1%TFE in DCM |
|
|
HMBA-MBHA Resin |
DIC/HOBt/DMAP |
60%TFA in DCM |
||
|
Peptide Amide |
Rink Amide Resin |
HOBt/HBTU/DIPEA |
95%TFA/Scavenger |
|
|
Rink Amide-MBHA Resin |
HOBt/HBTU/DIPEA |
95%TFA/Scavenger |
||
|
Rink Amide-AM Resin |
HOBt/HBTU/DIPEA |
95%TFA/Scavenger |
||
|
HMBA-MBHA Resin |
DIC/HOBt/DMAP |
Amine |
||
|
Protected Peptide Amide |
Sieber Resin |
HOBt/HBTU/DIPEA |
1% TFA in DCM |
|
|
Peptide Ester |
HMBA-MBHA Resin |
DIC/HOBt/DMAP |
ROH/DIEA/DMF |
|
|
Peptide Hydrazide |
HMBA-MBHA Resin |
DIC/HOBt/DMAP |
Hydrazine/DMF |
|
|
Hydrazine-2-Chlorotrityl Resin |
DIC/HOBt |
1% to 5% TFA in DCM |
Resins for Boc SPPS
|
Products |
Resins |
Attachment |
Cleavage |
|
|
Peptide Acid |
Merrifield Resin |
Cesium salt or KF |
HF or TFMSA |
|
|
Hydroxymethyl Resin |
DIC/HOBt/DMAP |
HFor TFMSA |
||
|
PAM Resin |
DIC/HOBt/DMAP |
HFor TFMSA |
||
|
Protected Peptide Acid |
Oxime Resin |
DIC/HOBt/DMAP |
NaOH/Dioxane |
|
|
Peptide Amide |
MBHA Resin |
DIC/HOBt |
HF or TFMSA |
|
|
BHA Resin |
DIC/HOBt |
HF |
||
|
Peptide Hydrazide |
Oxime Resin |
DIC/HOBt/DMAP |
Hydrazine/ DMF |
|
|
Peptide Ester |
Oxime Resin |
DIC/HOBt/DMAP |
MeOH/DMF/TEA |
Bead Size Conversion Table
Mesh | µm | Mesh | µm | Mesh | µm | |
20 | 850 | 60 | 250 | 200 | 75 | |
25 | 710 | 70 | 212 | 325 | 45 | |
35 | 500 | 100 | 150 | 400 | 38 | |
50 | 300 | 140 | 106 |
