| Synonym: | 4-Hydroxymethyl-phenylacetamidomethyl Resin |
| Cross Linker (DVB): | 1% |
| Particle Size (mesh): | 200-400 |
| Loading (mmol/g): | 1.0-1.6 |
| PAM resin has become the most widely used resin in Boc chemistry peptide synthesis. It has greater acid stability than Merrifield resin, making it suitable for synthesizing medium and large peptides [1], such as DNA binding polyamines [2], human matrix Gla proteen(MGP) [3], Kappa-casein [4]. The first carboxylic acid is attached to Pam resin using an activating agent such as dicyclohexylcarbodiimide(DCC) and a catalytic amount of 4-dimethylaminopridine(DMAP) [5]. These conditions can lead to partial epimerization of the amino acids, so HOBt is normally added to reduce racemization [6]. References [1] B. Gutte & R. B. Merrifield, J. Biol. Chem., 1971, 246, 1922 [2] J. M. Belitsky, et al., Bioorg. Med. Chem., 2002, 10(8), 2767 [3] T. M. Hackeng, et al., Protein Sci., 2001, 10, 864 [4] P. Bansal, et al., Austr. J. Dairy Technol., 2000, 55, 91 [5] M. Gairi, et al., Tetrahedron Lett., 1994, 35, 175 [6] E. Giralt, et al., Tetrahedron, 1981, 37, 2007 |
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