| Synonym: | 5-Carboxytetramethylrhodamine, succinimidyl ester |
| CAS #: | 150810-68-7 |
| Molecular Formula: | C29H25N3O7 |
| Molecular Weight: | 527.5 |
| 5-TAMRA, SE (λex/λem: 547/574nm in DMSO)and its conjugates yield bright, pH-insensitive orange-red fluorescence with good photostability. 5-TAMRA,SE is predominantly used for labeling peptides and proteins while 6-TAMRA, SE is often used for labeling nucleotides and sequencing nucleic acids. Although the mixed TAMRA isomers are predominantly used for labeling proteins, the single TAMRA isomers are increasingly preferred for labeling peptides and nucleotides because they give better resolution in HPLC purification that is often required in the conjugation processes. References 1. Evans NA, et al. (2001). Visualizing differences in ligand-induced beta-arrestin-GFP interactions and trafficking between three recently characterized G protein-coupled receptors. J Neurochem 77, 476-85. 2. Nasarabadi S, et al. (1999). Simultaneous detection of TaqMan probes containing FAM and TAMRA reporter fluorophores. Biotechniques 27, 1116-8. |
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