| Synonyms: | 5-Carboxyfluorescein, succinimidyl ester |
| CAS #: | 92557-80-7 |
| Molecular Formula: | C25H15NO9 |
| Molecular Weight: | 473.4 |
| 5-FAM, SE (λex/λem: 492/518nm in DMF) is the amine-reactive succinimidyl ester of single isomer 5-FAM acid. It is one of the most popular green fluorescent reagents used for labeling peptides, proteins and nucleotides. It has also been used to prepare various small fluorescent molecules. References 1. Sakamoto M, et al. (2003). Application of terminal RFLP analysis to characterize oral bacterial flora in saliva of healthy subjects and patients with periodontitis. J Med Microbiol 52, 79-89. 2. Hahn M, et al. (2001). Influence of fluorophore dye labels on the migration behavior of polymerase chain reaction-amplified short tandem repeats during denaturing capillary electrophoresis. Electrophoresis 22, 2691-700. 3.Araie, M., Carboxyfluorescein. A dye for evaluating the corneal endothelial barrier function in vivo. Exp Eye Res 1986, 42, 141-50. |
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