| Synonym: | N-(aminoethyl)-5-naphthylamine-1-sulfonic acid |
| CAS #: | 50402-56-7 |
| Molecular Formula: | C12H14N2O3S |
| Molecular Weight: | 266.3 |
| 1,5-EDANS (λex/λem: 335/493nm in DMSO) is a fluorescent reagent with a terminal amine for derivatization. It is one of the most popular donors for developing FRET-based nucleic acid probes and protease substrates. EDANS is often paired with DABCYL or DABSYL in FRET-based probes. 1,5-EDANS has similar spectral properties to the naphthalenesulfonic acids. Its fluorescence is environment-sensitive. References 1. Becker BF, et al. (2002). Application of peptides containing the cleavage sequence of pro-TNFa in assessing TACE activity of whole cells. Biol Chem 383, 1821-6. 2. Wang GT, et al. (1993). A continuous fluorescence assay of renin activity. Anal Biochem 210, 351-9. 3. Warfield, R., et al.: Org. Biomol. Chem., 4, 3626 (2006), Elston, C., et al.: Anal. Biochem., 368, 87 (2007). |
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