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6-TAMRA (λex/λem: 541/568nm in DMF or DMSO) is the other purified single isomer of 5(6)-TAMRA. It is predominantly used for oligonucleotide labeling and automated DNA sequencing applications.. It is also used in fluorescence in situ hybridization (FISH). References 1. Hsu TM, et al. (2001). Genotyping single-nucleotide polymorphisms by the invader assay with dual-color fluorescence polarization detection. Clin Chem 47, 1373-7. 2. Schutz E, et al. (2000). Genotyping of eight thiopurine methyltransferase mutations: three-color multiplexing, two-color/shared anchor and fluorescence-quenching hybridization probe assays based on thermodynamic nearest-neighbor probe design. Clin Chem 46, 1728-37. 3. Lyttle, M.H., et al., A tetramethyl rhodamine (tamra) phosphoramidite facilitates solid-phase-supported synthesis of 5'-tamra DNA. J Org Chem 2000, 65, 9033-8. |
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