6-ROX, SE (λex/λem: 575/602nm in DMSO) is the other purified single isomer of 5(6)-ROX, SE. 6-ROX is an amine-reactive form of carboxy-X-rhodamine and is widely used for oligonucleotide labeling and automated DNA sequencingapplications. ROX is very unstable. Cautions must be exercised to prevent the deterioration during storage.
1. Dominko T, et al. (2000). Dynamic imaging of the metaphase II spindle and maternal chromosomesin bovine oocytes: implications for enucleation efficiency verification, avoidance of parthenogenesis, and successful embryogenesis. Biol Reprod 62, 150-4.
2. Yoshikawa, Y., et al., Differential display with carboxy-x-rhodamine-labeled primers and the selection of differentially amplified cdna fragments without cloning. Anal Biochem 1998, 256, 82-91.
3. Hung, S.C., et al., Cyanine dyes with high absorption cross section as donor chromophores in energy transfer primers. Anal Biochem 1996, 243, 15-27.
4. Li, Y. and A.N. Glazer, Design, synthesis, and spectroscopic properties of peptide-bridged fluorescence energy-transfer cassettes. Bioconjug Chem 1999, 10, 241-5.