5-ROX, SE (λex/λem: 573/602nm in DMF) is a purified single isomer of 5(6)-ROX, SE. Single isomers of ROX are increasingly preferred for labeling peptides and nucleotides because they give better resolution in HPLC purifications that are often required in bioconjugations. Variable ratios of the 5- and 6-isomers often cause complications in the interpretation of labeling results and assay performances.
5-ROX is predominantly used to label peptides and proteins. 5-ROX, SE shows good reactivity and selectivity towards aliphatic amines.It has a very low reactivity towards aromatic amines, alcohols, phenols (including tyrosine) and histidine.It has all the essential components for performing the conjugation reaction and for purifying the conjugate.
Compared to other rhodamines, ROX is very unstable. Cautions must be exercised to prevent the deterioration during storage.
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