6-ROX (λex/λem: 570/591nm in DMF) is a derivative of carboxy-X-rhodamine, the pure-6 isomer of 5(6)-ROX. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-ROX and 6-ROX might significantly affect some biological properties of the underlying conjugates. 6-ROX is widely used to label nucleotides and for DNA sequencing; and also for specific applications of Real-Time PCR standard (free acid form), DNA sequencing, chromatography and CE analysis.
1. Seo, T.S., et al., Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides. Proc Natl Acad Sci USA 2005, 102, 5926-31.
2.Seo, T.S., et al., Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry. Proc Natl Acad Sci USA 2004, 101, 5488-93.
3.Lu H, et al. (1994). High-speed and high-accuracy DNA sequencing by capillary gel electrophoresis in a simple, low cost instrument. Two-color peak-height encoded sequencing at 40°C. J Chromatogr A 680, 497-501.
4.Carson, S, et al. (1993); Anal Chem 65, 3219-26
5. Slateva K, et al. (2001); Tissue Antigens 58, 250-4