Products > Fluorescent Dyes > 6-FAM


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500 mg
1 g
5 g

6-FAM (λex/λem: 495/517nm in DMSO or DMF pH > 9.0) contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. Complementary to 5-FAM isomer, 6-FAM is mainly used for labeling nucleotides and nucleic acids. 6-FAM has relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.

6-FAM plays a particularly important role in real-time PCR applications, being used as a reporter moiety in TaqMan probes, Scorpion primers and Molecular Beacons. For such probes, 6-FAM is most commonly paired with the dark quencher BHQ-1, as the two have excellent spectral overlap. 6-FAM-labeled primers have also been used for bacterial SNP genotyping by allele-specific real-time PCR.

6-FAM can be used to label DNA oligos for use as hybridization probes in a variety of in vivo and in vitro research or diagnostic applications, as well as for structure-function studies of DNA, RNA, and protein-oligonucleotide complexes. Oligos labeled with 6-FAM at the 5’-end can be used as PCR and DNA sequencing primers to generate fluorescently-labeled PCR, sequencing or genetic analysis (AFLP or microsatellite) products.


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