Products > Fluorescent Dyes>1,5-EDANS


1,5-EDANS
(N-(aminoethyl)-5-naphthylamine-1-sulfonic acid)



          CAS #:
50402-56-7
          Molecular Formula:
C12H14N2O3S
          Molecular Weight:
266.3
          Purity:
≥95%


Catalog #
Quantity
Price (US $)
240501
5 g
300.00
25 g
900.00

1,5-EDANS (λex/λem: 335/493nm in DMSO) is a fluorescent reagent with a terminal amine for derivatization. It is one of the most popular donors for developing FRET-based nucleic acid probes and protease substrates. EDANS is often paired with DABCYL or DABSYL in FRET-based probes.

1,5-EDANS has similar spectral properties to the naphthalenesulfonic acids. Its fluorescence is environment-sensitive.

References

1. Becker BF, et al. (2002). Application of peptides containing the cleavage sequence of pro-TNFa in assessing TACE activity of whole cells. Biol Chem 383, 1821-6.
2. Wang GT, et al. (1993). A continuous fluorescence assay of renin activity. Anal Biochem 210, 351-9.
3. Warfield, R., et al.: Org. Biomol. Chem., 4, 3626 (2006), Elston, C., et al.: Anal. Biochem., 368, 87 (2007).